this post was submitted on 02 Jul 2025
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Science Memes

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[โ€“] Contramuffin@lemmy.world 37 points 6 days ago (1 children)

Explanation: CRISPR-Cas9 is most commonly known to be a gene editor, but since its initial discovery, people have found that with some minor tweaks, CRISPR can be made to do a bunch of other things, things that biologists could have only dreamed of previously.

If you introduce a mutation that deactivates the Cas9 protein, the entire CRISPR complex still binds to DNA. Then, you can essentially glue whatever you want onto this modified Cas9 to produce a bunch of different effects:

  • deactivated Cas9, no additional modifications: blocks other proteins from touching the DNA, which suppresses a gene without destroying it
  • glue on some transcriptional activators: makes the cell express a gene
  • glue on some fluorescent proteins: makes your DNA glow, lets you see where it is

Well done! I zoomed in and felt dumb. But thanks to your succinct explanation, I now have a sweet Dunning-Kruger high